Reference - PMID:10365961 - The MAPK kinase Pek1 acts as a phosphorylation-dependent molecular switch.
Reference summary
- PubMed ID
- PMID:10365961
- Title
- The MAPK kinase Pek1 acts as a phosphorylation-dependent molecular switch.
- Authors
- Sugiura R, Toda T, Dhut S, Shuntoh H, Kuno T
- Citation
- Nature 1999 Jun 03;399(6735):479-83
- Publication year
- 1999
- Abstract
- The mitogen-activated protein kinase (MAPK) pathway is a highly conserved eukaryotic signalling cascade that converts extracellular signals into various outputs, such as cell growth and differentiation. MAPK is phosphorylated and activated by a specific MAPK kinase (MAPKK): MAPKK is therefore considered to be an activating regulator of MAPK. Pmk1 is a MAPK that regulates cell integrity and which, with calcineurin phosphatase, antagonizes chloride homeostasis in fission yeast. We have now identified Pek1, a MAPKK for Pmk1 MAPK. We show here that Pek1, in its unphosphorylated form, acts as a potent negative regulator of Pmk1 MAPK signalling. Mkh1, an upstream MAPKK kinase (MAPKKK), converts Pek1 from being an inhibitor to an activator. Our results indicate that Pek1 has a dual stimulatory and inhibitory function which depends on its phosphorylation state. This switch-like mechanism could contribute to the all-or-none physiological response mediated by the MAPK signalling pathway.
