Reference - PMID:10373519 - A Uve1p-mediated mismatch repair pathway in Schizosaccharomyces pombe.
Reference summary
- PubMed ID
- PMID:10373519
- Title
- A Uve1p-mediated mismatch repair pathway in Schizosaccharomyces pombe.
- Authors
- Kaur B, Fraser JL, Freyer GA, Davey S, Doetsch PW
- Citation
- Mol Cell Biol 1999 Jul;19(7):4703-10
- Publication year
- 1999
- Abstract
- UV damage endonuclease (Uve1p) from Schizosaccharomyces pombe was initially described as a DNA repair enzyme specific for the repair of UV light-induced photoproducts and proposed as the initial step in an alternative excision repair pathway. Here we present biochemical and genetic evidence demonstrating that Uve1p is also a mismatch repair endonuclease which recognizes and cleaves DNA 5' to the mispaired base in a strand-specific manner. The biochemical properties of the Uve1p-mediated mismatch endonuclease activity are similar to those of the Uve1p-mediated UV photoproduct endonuclease. Mutants lacking Uve1p display a spontaneous mutator phenotype, further confirming the notion that Uve1p plays a role in mismatch repair. These results suggest that Uve1p has a surprisingly broad substrate specificity and may function as a general type of DNA repair protein with the capacity to initiate mismatch repair in certain organisms.
