Reference - PMID:10559981 - A Rad3-Rad26 complex responds to DNA damage independently of other checkpoint proteins.
Reference summary
- PubMed ID
- PMID:10559981
- Title
- A Rad3-Rad26 complex responds to DNA damage independently of other checkpoint proteins.
- Authors
- Edwards RJ, Bentley NJ, Carr AM
- Citation
- Nat Cell Biol 1999 Nov;1(7):393-8
- Publication year
- 1999
- Abstract
- The conserved PIK-related kinase Rad3 is required for all DNA-integrity-checkpoint responses in fission yeast. Here we report a stable association between Rad3 and Rad26 in soluble protein extracts. Rad26 shows Rad3-dependent phosphorylation after DNA damage. Unlike phosphorylation of Hus1, Crb2/Rhp9, Cds1 and Chk1, phosphorylation of Rad26 does not require other known checkpoint proteins. Rad26 phosphorylation is the first biochemical marker of Rad3 function, indicating that Rad3-related checkpoint kinases may have a direct role in DNA-damage recognition.
