Reference - PMID:10747036 - A novel SMC protein complex in Schizosaccharomyces pombe contains the Rad18 DNA repair protein.
Reference summary
- PubMed ID
- PMID:10747036
- Title
- A novel SMC protein complex in Schizosaccharomyces pombe contains the Rad18 DNA repair protein.
- Authors
- Fousteri MI, Lehmann AR
- Citation
- EMBO J 2000 Apr 03;19(7):1691-702
- Publication year
- 2000
- Abstract
- In Schizosaccharomyces pombe, rad18 is an essential gene involved in the repair of DNA damage produced by ionizing radiation and in tolerance of UV-induced DNA damage. The Rad18 protein is a member of the SMC (structural maintenance of chromosomes) superfamily, and we show that, like the other SMC proteins in condensin and cohesin, Rad18 is a component of a high-molecular-weight complex. This complex contains at least six other proteins, the largest of which is Spr18, a novel SMC family member closely related to Rad18, and likely to be its heterodimeric partner. SMC proteins have ATP-binding domains at the N- and C-termini, and two extended coiled-coil domains separated by a hinge in the middle. We show that the N-terminal ATP-binding domain of Rad18 is essential for all functions, and overexpression of an N-terminal mutant has a dominant-negative effect. We have identified an important mutation (S1045A) near the C-terminus of Rad18 that separates its repair and essential roles. Potential models for the role of the Rad18-Spr18 complex during DNA repair are discussed.
