Reference - PMID:10864871 - Requirement of Mis6 centromere connector for localizing a CENP-A-like protein in fission yeast.

Reference summary

PubMed ID

PMID:10864871

Title

Requirement of Mis6 centromere connector for localizing a CENP-A-like protein in fission yeast.

Authors

Takahashi K, Chen ES, Yanagida M

Citation

Science 2000 Jun 23;288(5474):2215-9

Publication year

2000

Abstract

Mammalian kinetochores contain the centromere-specific histone H3 variant CENP-A, whose incorporation into limited chromosomal regions may be important for centromere function and chromosome segregation during mitosis. However, regulation of CENP-A localization and its role have not been clear. Here we report that the fission yeast homolog SpCENP-A is essential for establishing centromere chromatin associated with equal chromosome segregation. SpCENP-A binding to the nonrepetitious inner centromeres depended on Mis6, an essential centromere connector protein acting during G1-S phase of the cell cycle. Mis6 is likely required for recruiting SpCENP-A to form proper connection of sister centromeres.

Annotation