Reference - PMID:10871341 - Isolation of an essential Schizosaccharomyces pombe gene, prp31(+), that links splicing and meiosis.
Reference summary
- PubMed ID
- PMID:10871341
- Title
- Isolation of an essential Schizosaccharomyces pombe gene, prp31(+), that links splicing and meiosis.
- Authors
- Bishop DT, McDonald WH, Gould KL, Forsburg SL
- Citation
- Nucleic Acids Res 2000 Jun 01;28(11):2214-20
- Publication year
- 2000
- Abstract
- We carried out a screen for mutants that arrest prior to premeiotic S phase. One of the strains we isolated contains a temperature-sensitive allele mutation in the fission yeast prp31(+) gene. The prp31-E1 mutant is defective in vegetative cell growth and in meiotic progression. It is synthetically lethal with prp6 and displays a pre-mRNA splicing defect at the restrictive temperature. We cloned the wild-type gene by complementation of the temperature-sensitive mutant phenotype. Prp31p is closely related to human and budding yeast PRP31 homologs and is likely to function as a general splicing factor in both vegetative growth and sexual differentiation.
