Reference - PMID:10879493 - Identification and characterization of a novel gene, hos3+, the function of which is necessary for growth under high osmotic stress in fission yeast.
Reference summary
- PubMed ID
- PMID:10879493
- Title
- Identification and characterization of a novel gene, hos3+, the function of which is necessary for growth under high osmotic stress in fission yeast.
- Authors
- Aoyama K, Kawaura R, Yamada H, Aiba H, Mizuno T
- Citation
- Biosci Biotechnol Biochem 2000 May;64(5):1099-102
- Publication year
- 2000
- Abstract
- hos3 mutants of the fission yeast Schizosaccharomyces pombe showed the phenotype of high osmolarity sensitivity for growth. An S. pombe strain carrying the hos3-M26 allele cannot form colonies on agar plates containing 2 M glucose, but the parental strain can do so very well, as demonstrated previously. The hos3+ gene was cloned and identified as one that encodes a small protein of 94 amino acids, which shows no sequence similarity to any other proteins in the current databases. A hos3delta strain, which we then constructed, had the phenotype of high osmolarity sensitivity, as in the case of the original hos3-M26 mutant. More interestingly, when these hos- cells were grown in the non-permissive growth condition in the presence of 2 M glucose, we found that unusually many septated cells were accumulated after a prolonged incubation. A multicopy suppressor gene for hos- mutations was also isolated and identified as the dsk1+ gene encoding a protein kinase, which was previously suggested to be implicated in a process of the mitotic regulation of S. pombe. The function of the hos3+ gene is discussed from these results.
