Reference - PMID:10978320 - The splicing factor, Prp40, binds the phosphorylated carboxyl-terminal domain of RNA polymerase II.
Reference summary
- PubMed ID
- PMID:10978320
- Title
- The splicing factor, Prp40, binds the phosphorylated carboxyl-terminal domain of RNA polymerase II.
- Authors
- Morris DP, Greenleaf AL
- Citation
- J Biol Chem 2000 Dec 22;275(51):39935-43
- Publication year
- 2000
- Abstract
- We showed previously that the WW domain of the prolyl isomerase, Ess1, can bind the phosphorylated carboxyl-terminal domain (phospho-CTD) of the largest subunit of RNA Polymerase II. Analysis of phospho-CTD binding by four other WW domain-containing Saccharomyces cerevisiae proteins indicates the splicing factor, Prp40, and the RNA polymerase II ubiquitin ligase, Rsp5, can also bind the phospho-CTD. The identification of Prp40 as a phospho-CTD binding protein represents the first demonstration of direct interaction between a documented splicing factor and the phospho-CTD. Domain dissection studies reveal that phospho-CTD binding occurs at multiple locations in Prp40, including sites in both the WW and FF domain regions. Because the conserved repeats of the CTD make it an ideal ligand for multi-site binding events, the implications of multi-site binding are discussed. Our data suggest a mechanism by which the phospho-CTD of elongating RNA polymerase II facilitates commitment complex formation by juxtaposing the 5' and 3' splice sites.
