Reference - PMID:11270571 - A novel genetic screen identifies checkpoint-defective alleles of Schizosaccharomyces pombe chk1.
Reference summary
- PubMed ID
- PMID:11270571
- Title
- A novel genetic screen identifies checkpoint-defective alleles of Schizosaccharomyces pombe chk1.
- Authors
- Wan S, Walworth NC
- Citation
- Curr Genet 2001 Jan;38(6):299-306
- Publication year
- 2001
- Abstract
- The protein kinase Chk1 is required in the fission yeast Schizosaccharomyces pombe for delaying cell cycle progression in response to DNA damage. Chk1 becomes phosphorylated when DNA is damaged by a variety of agents, including the anti-tumor drug camptothecin. To further characterize the behavior of Chk1 in response to DNA damage, we used PCR-based mutagenesis of the chk1 gene coupled with in vivo gap repair to generate mutant alleles. Of 44 chk1 mutants recovered, six encode full-length proteins that confer a DNA damage-sensitive phenotype. All of the alleles render cells checkpoint-defective, but confer subtle differences in sensitivity to camptothecin or UV light. Mutant alleles were sequenced and served to identify regions of the protein that are critical for checkpoint function.
