Reference - PMID:11283354 - Role of histone H3 lysine 9 methylation in epigenetic control of heterochromatin assembly.
Reference summary
- PubMed ID
- PMID:11283354
- Title
- Role of histone H3 lysine 9 methylation in epigenetic control of heterochromatin assembly.
- Authors
- Nakayama J, Rice JC, Strahl BD, Allis CD, Grewal SI
- Citation
- Science 2001 Apr 06;292(5514):110-3
- Publication year
- 2001
- Abstract
- The assembly of higher order chromatin structures has been linked to the covalent modifications of histone tails. We provide in vivo evidence that lysine 9 of histone H3 (H3 Lys9) is preferentially methylated by the Clr4 protein at heterochromatin-associated regions in fission yeast. Both the conserved chromo- and SET domains of Clr4 are required for H3 Lys9 methylation in vivo. Localization of Swi6, a homolog of Drosophila HP1, to heterochomatic regions is dependent on H3 Lys9 methylation. Moreover, an H3-specific deacetylase Clr3 and a beta-propeller domain protein Rik1 are required for H3 Lys9 methylation by Clr4 and Swi6 localization. These data define a conserved pathway wherein sequential histone modifications establish a "histone code" essential for the epigenetic inheritance of heterochromatin assembly.
