Reference - PMID:12390246 - Cut1/separase C-terminus affects spindle pole body positioning in interphase of fission yeast: pointed nuclear formation.
Reference summary
- PubMed ID
- PMID:12390246
- Title
- Cut1/separase C-terminus affects spindle pole body positioning in interphase of fission yeast: pointed nuclear formation.
- Authors
- Nakamura T, Nagao K, Nakaseko Y, Yanagida M
- Citation
- Genes Cells 2002 Nov;7(11):1113-24
- Publication year
- 2002
- Abstract
- The separase-securin complex is required for anaphase. Separase activated by securin destruction cleaves the cohesin subunit Scc1/Rad21 enriched in kinetochores. Fission yeast Cut1/separase resides in interphase cytoplasm and mobilizes to the spindle and the spindle pole bodies (SPBs) in mitosis, while Cut2/securin remains in the nucleus from interphase to metaphase, and temporarily locates at the short spindle.
We here report a novel SPB-led dynamic nuclear movement in fission yeast, when the Cut1 C-terminal fragment is over-expressed. The tip of the pointed nucleus contained both SPB and centromeric DNA, and rapidly moved along the bundled cytoplasmic microtubules. The same pointed nucleus was produced when the human separase C-fragment was over-expressed. The pointed nuclear formation did not require the protease site of separase, but required the conserved C-terminus and a microtubule- and kinetochore-binding protein Mtc1/Alp14, a homologue of frog XMAP215 and budding yeast Stu2. The movement-inducing C-fragment should be cytoplasmic, as the pointed nucleus was abolished when the fragment contained the NLS (nuclear localization signal).
Overproduced separase C-fragment abolishes correct SPB-positioning in interphase. Resulting pointed nuclear formation (alternatively called 'pigtail movement') requires cytoplasmic microtubules and Mtc1/Alp14.
