Reference - PMID:12471450 - Activation of the urease of Schizosaccharomyces pombe by the UreF accessory protein from soybean.
Reference summary
- PubMed ID
- PMID:12471450
- Title
- Activation of the urease of Schizosaccharomyces pombe by the UreF accessory protein from soybean.
- Authors
- Bacanamwo M, Witte CP, Lubbers MW, Polacco JC
- Citation
- Mol Genet Genomics 2002 Dec;268(4):525-34
- Publication year
- 2002
- Abstract
- Plant orthologs of the bacterial urease accessory genes ureD and ureF, which are required for the insertion of the nickel ion at the active site, have been isolated from soybean ( Glycine max L. Merr.), tomato ( Lycopersicon esculentum) and Arabidopsis thaliana. The functionality of soybean UreD and UreF was tested by measuring their ability to complement urease-negative mutants of Schizosaccharomyces pombe, a eukaryote which produces a "plant-like" urease of ~90 kDa. The S. pombe ure4 mutant was complemented by a 12-kb fragment of S. pombe genomic DNA, which was shown by PCR to contain a putative ureD gene. However, ure4 was not complemented by a UreD cDNA soybean, expressed under the control of a strong promoter. In contrast, an S. pombe ure3 mutation was complemented by both a 10-kb fragment of S. pombe DNA containing ureF and the UreF cDNA from soybean. Soybean Eu2 is a candidate urease accessory gene; its product cooperates with the Eu3 protein in activating apourease in vitro. However, the sequences of UreD and UreF transcripts from two eu2/eu2 mutants, recovered as RT-PCR products, revealed no mutational alteration, suggesting that Eu2 encodes neither UreD nor UreF.
