Reference - PMID:12482946 - Dicer is required for chromosome segregation and gene silencing in fission yeast cells.
Reference summary
- PubMed ID
- PMID:12482946
- Title
- Dicer is required for chromosome segregation and gene silencing in fission yeast cells.
- Authors
- Provost P, Silverstein RA, Dishart D, Walfridsson J, Djupedal I, Kniola B, Wright A, Samuelsson B, Radmark O, Ekwall K
- Citation
- Proc Natl Acad Sci U S A 2002 Dec 24;99(26):16648-53
- Publication year
- 2002
- Abstract
- RNA interference is a form of gene silencing in which the nuclease Dicer cleaves double-stranded RNA into small interfering RNAs. Here we report a role for Dicer in chromosome segregation of fission yeast. Deletion of the Dicer (dcr1+) gene caused slow growth, sensitivity to thiabendazole, lagging chromosomes during anaphase, and abrogated silencing of centromeric repeats. As Dicer in other species, Dcr1p degraded double-stranded RNA into approximately 23 nucleotide fragments in vitro, and dcr1Delta cells were partially rescued by expression of human Dicer, indicating evolutionarily conserved functions. Expression profiling demonstrated that dcr1+ was required for silencing of two genes containing a conserved motif.
