Reference - PMID:1356078 - Thialysine-resistant mutants and uptake of lysine in Schizosaccharomyces pombe.
Reference summary
- PubMed ID
- PMID:1356078
- Title
- Thialysine-resistant mutants and uptake of lysine in Schizosaccharomyces pombe.
- Authors
- Sychrová H, Chevallier MR, Horák J, Kotyk A
- Citation
- Curr Genet 1992 Apr;21(4-5):351-5
- Publication year
- 1992
- Abstract
- Mutants defective in lysine transport were isolated and characterized. After UV-mutagenesis colonies resistant to thialysine, a toxic analogue of lysine, were isolated and L-lysine uptake into the mutant strains was analyzed. Among the thialysine-resistant strains a group of mutants was found, where the half-saturation constant, KT, of the high-affinity transport system for lysine was higher than in the wild-type, the high-affinity transport system for basic amino acids being specifically affected. This was confirmed by a complementation test in which all the thialysine-resistant strains with a higher KT for lysine uptake belonged to one complementation group. Kinetic and genetic analysis showed that our mutants were identical with can1-1 mutants, showing that a single high-affinity system for the transport of basic amino acids exists in S. pombe.
