Reference - PMID:15601865 - Structural and functional analysis of essential pre-mRNA splicing factor Prp19p.
Reference summary
- PubMed ID
- PMID:15601865
- Title
- Structural and functional analysis of essential pre-mRNA splicing factor Prp19p.
- Authors
- Ohi MD, Vander Kooi CW, Rosenberg JA, Ren L, Hirsch JP, Chazin WJ, Walz T, Gould KL
- Citation
- Mol Cell Biol 2005 Jan;25(1):451-60
- Publication year
- 2005
- Abstract
- U-box-containing Prp19p is an integral component of the Prp19p-associated complex (the nineteen complex, or NTC) that is essential for activation of the spliceosome. Prp19p makes numerous protein-protein contacts with other NTC components and is required for NTC stability. Here we show that Prp19p forms a tetramer in vitro and in vivo and we map the domain required for its oligomerization to a central tetrameric coiled-coil. Biochemical and in vivo analyses are consistent with Prp19p tetramerization providing an interaction surface for a single copy of its binding partner, Cef1p. Electron microscopy showed that the isolated Prp19p tetramer is an elongated particle consisting of four globular WD40 domains held together by a central stalk consisting of four N-terminal U-boxes and four coiled-coils. These structural and functional data provide a basis for understanding the role of Prp19p as a key architectural component of the NTC.
