Reference - PMID:15689108 - Ferrichrome in Schizosaccharomyces pombe--an iron transport and iron storage compound.
Reference summary
- PubMed ID
- PMID:15689108
- Title
- Ferrichrome in Schizosaccharomyces pombe--an iron transport and iron storage compound.
- Authors
- Schrettl M, Winkelmann G, Haas H
- Citation
- Biometals 2004 Dec;17(6):647-54
- Publication year
- 2004
- Abstract
- Schizosaccharomyces pombe has been assumed not to produce siderophores. Nevertheless, the genomic sequence of this fission yeast revealed the presence of siderophore biosynthetic genes for hydroxamates. Applying a bioassay based on an Aspergillus nidulans strain deficient in siderophore biosynthesis, and using reversed-phase HPLC and mass spectrometry analysis, we demonstrate that S. pombe excretes and accumulates intracellularly the hydroxamate-type siderophore ferrichrome. Under iron-limiting conditions, the cellular ferrichrome pool was present in the desferri-form, while under iron-richconditions, in the ferri-form. In contrast to S. pombe, hydroxamate-type siderophores could not be detected intwo other yeast species, Saccharomyces cerevisiae and Candida albicans.
