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Reference - PMID:15797925 - The nuclear rim protein Amo1 is required for proper microtubule cytoskeleton organisation in fission yeast.

Reference summary

PubMed ID
PMID:15797925
Title
The nuclear rim protein Amo1 is required for proper microtubule cytoskeleton organisation in fission yeast.
Authors
Pardo M, Nurse P
Citation
J Cell Sci 2005 Apr 15;118(Pt 8):1705-14
Publication year
2005
Abstract
Microtubules have a central role in cell division and cell polarity in eukaryotic cells. The fission yeast is a useful organism for studying microtubule regulation owing to the highly organised nature of its microtubular arrays. To better understand microtubule dynamics and organisation we carried out a screen that identified over 30 genes whose overexpression resulted in microtubule cytoskeleton abnormalities. Here we describe a novel nucleoporin-like protein, Amo1, identified in this screen. Amo1 localises to the nuclear rim in a punctate pattern that does not overlap with nuclear pore complex components. Amo1Delta cells are bent, and they have fewer microtubule bundles that curl around the cell ends. The microtubules in amo1Delta cells have longer dwelling times at the cell tips, and grow in an uncoordinated fashion. Lack of Amo1 also causes a polarity defect. Amo1 is not required for the microtubule loading of several factors affecting microtubule dynamics, and does not seem to be required for nuclear pore function.

Annotation

GO cellular component

GO:0034399 - nuclear periphery

Genes:

GO:0044732 - mitotic spindle pole body

Genes:

Multi-locus phenotype

FYPO:0002459 - viable branched, elongated vegetative cell

Genes:

Genotypes:

Single locus phenotype

FYPO:0001418 - abnormal microtubule cytoskeleton morphology during vegetative growth

Genes:

Genotypes:

FYPO:0000338 - abnormal mitotic spindle

Genes:

Genotypes:

FYPO:0001584 - abnormal protein localization to cell tip during vegetative growth

Genes:

Genotypes:

FYPO:0001394 - activation of monopolar cell growth at either end

Genes:

Genotypes:

FYPO:0003202 - actomyosin contractile ring contraction uncoupled from septum assembly

Genes:

Genotypes:

FYPO:0003327 - curved interphase microtubules

Genes:

Genotypes:

FYPO:0004862 - curved microtubules

Genes:

Genotypes:

FYPO:0004702 - cytoplasmic microtubules absent from cell

Genes:

Genotypes:

FYPO:0003306 - decreased mitotic index

Genes:

Genotypes:

FYPO:0003150 - decreased NETO

Genes:

Genotypes:

FYPO:0003208 - decreased protein localization to cell tip, with protein distributed in plasma membrane or cortex

Genes:

Genotypes:

FYPO:0001355 - decreased vegetative cell population growth

Genes:

Genotypes:

FYPO:0003417 - increased microtubule dwell time at cell tip

Genes:

Genotypes:

FYPO:0002061 - inviable vegetative cell population

Genes:

Genotypes:

FYPO:0005880 - long interphase microtubules curved around cell end

Genes:

Genotypes:

FYPO:0002818 - microtubule bundles present in decreased numbers

Genes:

Genotypes:

FYPO:0002004 - microtubules absent from cell

Genes:

Genotypes:

FYPO:0002071 - mislocalized nucleus during vegetative growth

Genes:

Genotypes:

FYPO:0000339 - mislocalized septum during vegetative growth

Genes:

Genotypes:

FYPO:0003328 - misoriented interphase microtubules

Genes:

Genotypes:

FYPO:0000276 - monopolar mitotic spindle

Genes:

Genotypes:

FYPO:0005960 - multipolar mitotic spindle

Genes:

Genotypes:

FYPO:0001686 - normal growth on carbendazim

Genes:

Genotypes:

FYPO:0000964 - normal growth on thiabendazole

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Genotypes:

FYPO:0003702 - normal microtubule cytoskeleton morphology during vegetative growth

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Genotypes:

FYPO:0000771 - normal nuclear pore localization

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FYPO:0004121 - normal protein import into nucleus during vegetative growth

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FYPO:0005707 - normal protein localization to eMTOC

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FYPO:0003185 - normal protein localization to microtubule cytoskeleton during mitotic interphase

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FYPO:0002966 - normal protein localization to mitotic spindle

Genes:

Genotypes:

FYPO:0002967 - normal protein localization to mitotic spindle pole body

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FYPO:0003193 - normal rate of microtubule depolymerization during vegetative growth

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FYPO:0003225 - normal rate of microtubule polymerization during vegetative growth

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FYPO:0004025 - resistance to carbendazim

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FYPO:0000069 - resistance to thiabendazole

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FYPO:0000091 - sensitive to thiabendazole

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FYPO:0003305 - separate mitotic half spindles present

Genes:

Genotypes:

FYPO:0001873 - shmoo formation in absence of opposite mating type

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Genotypes:

FYPO:0000732 - short bipolar mitotic spindle

Genes:

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FYPO:0002760 - short cytoplasmic microtubules

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Genotypes:

FYPO:0002400 - single microtubule bundle during mitotic interphase

Genes:

Genotypes:

FYPO:0001234 - slow vegetative cell population growth

Genes:

Genotypes:

FYPO:0004236 - thin mitotic spindle midzone

Genes:

Genotypes:

FYPO:0002459 - viable branched, elongated vegetative cell

Genes:

Genotypes:

FYPO:0002215 - viable curved elongated vegetative cell

Genes:

Genotypes:

FYPO:0002112 - viable curved vegetative cell

Genes:

Genotypes:

FYPO:0001492 - viable elongated vegetative cell

Genes:

Genotypes:

FYPO:0000648 - viable small vegetative cell

Genes:

Genotypes:

FYPO:0002060 - viable vegetative cell population

Genes:

Genotypes:

FYPO:0002177 - viable vegetative cell with normal cell morphology

Genes:

Genotypes: