Reference - PMID:15862318 - cda1+, encoding chitin deacetylase is required for proper spore formation in Schizosaccharomyces pombe.

Reference summary

PubMed ID

PMID:15862318

Title

cda1+, encoding chitin deacetylase is required for proper spore formation in Schizosaccharomyces pombe.

Authors

Matsuo Y, Tanaka K, Matsuda H, Kawamukai M

Citation

FEBS Lett 2005 May 09;579(12):2737-43

Publication year

2005

Abstract

In Schizosaccharomyces pombe, a major role of chitin is to build up a complete spore. Here, we analyzed the cda1(+) gene (SPAC19G12.03), which encodes a protein homologous to chitin deacetylases, to know whether it is required for spore formation in S. pombe. The homothallic Deltacda1 strain constructed by homologous recombination was found to form a little amount of abnormal spores that contained one, two, or three asci, similar to (but not as strong as) the phenotype observed in a deletion mutant of chs1 encoding chitin synthase 1. This phenotype is reversed by expression of S. cerevisiae chitin deacetylase CDA1 or CDA2, suggesting that cda1 encodes a chitin deacetylase. To support the role of Cda1 in sporulation, the timing of expression of cda1(+) mRNA increased during sporulation process. We also found that the Cda1 protein self-associated when its binding was tested both by two-hybrid system and immunoprecipitation. Thus, these data indicated that cda1(+) is required for proper spore formation in S. pombe.

Annotation