Reference - PMID:16444015 - Nickel resistance in fission yeast associated with the magnesium transport system.
Reference summary
- PubMed ID
- PMID:16444015
- Title
- Nickel resistance in fission yeast associated with the magnesium transport system.
- Authors
- Sarikaya AT, Akman G, Temizkan G
- Citation
- Mol Biotechnol 2006 Feb;32(2):139-46
- Publication year
- 2006
- Abstract
- We isolated and characterized a nickel (Ni2+)-resistant mutant (GA1) of Schizosaccharomyces pombe. This mutant strain displayed resistance to both Ni2+ and Zn2+, but not to Cd2+, Co2+, and Cu2+. The growth rate of GA1 increased proportionally with increasing Mg2+ concentrations until 50 mM Mg2+. The GA1 mutation phenotype suggests a defect in Mg2+ uptake. Sequence analysis of the GA1 open reading frame (ORF) O13779, which is homologous to the prokaryotic and eukaryotic CorA Mg2+ transport systems, revealed a point mutation at codon 153 (ccc to acc) resulting in a Pro153Thr substitution in the N-terminus of the CorA domain. Our results provide novel genetic information about Ni2+ resistance in fission yeast. Specifically, that reducing Mg2+ influx through the CorA Mg2+ transport membrane protein confers Ni2+ resistance in S. pombe. We also report that Ni2+ ion detoxification of the fission yeast is related to histidine metabolism and pH.
