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Reference - PMID:17248775 - Genetic Mapping in SCHIZOSACCHAROMYCES POMBE by Mitotic and Meiotic Analysis and Induced Haploidization.

Reference summary

PubMed ID
PMID:17248775
Title
Genetic Mapping in SCHIZOSACCHAROMYCES POMBE by Mitotic and Meiotic Analysis and Induced Haploidization.
Authors
Kohli J, Hottinger H, Munz P, Strauss A, Thuriaux P
Citation
Genetics 1977 Nov;87(3):471-89
Publication year
1977
Abstract
The genetic maps of the fission yeast Schizosaccharomyces pombe were extended through the use of haploidization (spontaneous or induced by m-fluorophenylalanine), as well as by tetrad, random spore and mitotic analysis. A new diploidization method utilizing a meiosis-deficient mutant and improved haploidization techniques was employed. As a result of these and previous studies, 118 genetic markers have been assigned to 3 linkage groups. Centromere markers for all 3 chromosomes were identified and genetic maps containing a total of 71 genes were constructed. Our experiments indicate that 3 is very likely to be the haploid chromosome number of S. pombe .

Annotation

GO biological process

GO:0006189 - 'de novo' IMP biosynthetic process

Genes:

GO:0006526 - L-arginine biosynthetic process

Genes:

GO:0000105 - L-histidine biosynthetic process

Genes:

GO:0009098 - L-leucine biosynthetic process

Genes:

GO:0055129 - L-proline biosynthetic process

Genes:

GO:0000162 - L-tryptophan biosynthetic process

Genes:

GO:0006571 - L-tyrosine biosynthetic process

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Single locus phenotype

FYPO:0000747 - growth auxotrophic for adenine

Genes:

Genotypes:

FYPO:0000035 - growth auxotrophic for arginine

Genes:

Genotypes:

FYPO:0005877 - growth auxotrophic for glutamate

Genes:

Genotypes:

FYPO:0002068 - growth auxotrophic for histidine

Genes:

Genotypes:

FYPO:0000635 - growth auxotrophic for leucine

Genes:

Genotypes:

FYPO:0000039 - growth auxotrophic for lysine

Genes:

Genotypes:

FYPO:0000040 - growth auxotrophic for methionine

Genes:

Genotypes:

FYPO:0005875 - growth auxotrophic for proline

Genes:

Genotypes:

FYPO:0005878 - growth auxotrophic for tryptophan

Genes:

Genotypes:

FYPO:0005876 - growth auxotrophic for tyrosine

Genes:

Genotypes:

FYPO:0001012 - growth auxotrophic for uracil

Genes:

Genotypes:

FYPO:0002705 - sensitive to methionine

Genes:

Genotypes: