Reference - PMID:1737756 - A DNA exonuclease induced during meiosis of Schizosaccharomyces pombe.
Reference summary
- PubMed ID
- PMID:1737756
- Title
- A DNA exonuclease induced during meiosis of Schizosaccharomyces pombe.
- Authors
- Szankasi P, Smith GR
- Citation
- J Biol Chem 1992 Feb 15;267(5):3014-23
- Publication year
- 1992
- Abstract
- In meiotic cells of the fission yeast Schizosaccharomyces pombe, a DNA exonuclease activity increased approximately 5-fold after premeiotic S-phase and decreased to the initial level before the meiotic divisions. We have purified this activity, designated exonuclease I, to near homogeneity. The activity co-purified with a polypeptide with an apparent molecular weight of 36,000. With a linear double-stranded DNA substrate, exonuclease I degraded only the 5'-ended strand from each end to produce 3'-single-stranded tails. The enzyme also acted on nicked circular DNA with comparable affinity. The meiotic induction of exonuclease I and its mode of action, similar to that of recombination-promoting exonucleases from bacteria, suggest that exonuclease I is involved in meiotic homologous recombination in S. pombe.
