Reference - PMID:18165685 - Pathway of actin filament branch formation by Arp2/3 complex.
Reference summary
- PubMed ID
- PMID:18165685
- Title
- Pathway of actin filament branch formation by Arp2/3 complex.
- Authors
- Beltzner CC, Pollard TD
- Citation
- J Biol Chem 2008 Mar 14;283(11):7135-44
- Publication year
- 2008
- Abstract
- A spectroscopic assay using pyrene-labeled fission yeast Arp2/3 complex revealed that the complex binds to and dissociates from actin filaments extremely slowly with or without the nucleation-promoting factor fission yeast Wsp1-VCA. Wsp1-VCA binds both Arp2/3 complex and actin monomers with high affinity. These two ligands have only modest impacts on the interaction of the other ligand with VCA. Simulations of a mathematical model based on the kinetic parameters determined in this study and elsewhere account for the full time course of actin polymerization in the presence of Arp2/3 complex and Wsp1-VCA and show that an activation step, postulated to follow binding of a ternary complex of Arp2/3 complex, a bound nucleation-promoting factor, and an actin monomer to an actin filament, has a rate constant at least 0.15 s(-1). Kinetic parameters determined in this study constrain the process of actin filament branch formation during cellular motility to one main pathway.
