Reference - PMID:18723894 - Rtf1-mediated eukaryotic site-specific replication termination.
Reference summary
- PubMed ID
- PMID:18723894
- Title
- Rtf1-mediated eukaryotic site-specific replication termination.
- Authors
- Eydmann T, Sommariva E, Inagawa T, Mian S, Klar AJ, Dalgaard JZ
- Citation
- Genetics 2008 Sep;180(1):27-39
- Publication year
- 2008
- Abstract
- The molecular mechanisms mediating eukaryotic replication termination and pausing remain largely unknown. Here we present the molecular characterization of Rtf1 that mediates site-specific replication termination at the polar Schizosaccharomyces pombe barrier RTS1. We show that Rtf1 possesses two chimeric myb/SANT domains: one is able to interact with the repeated motifs encoded by the RTS1 element as well as the elements enhancer region, while the other shows only a weak DNA binding activity. In addition we show that the C-terminal tail of Rtf1 mediates self-interaction, and deletion of this tail has a dominant phenotype. Finally, we identify a point mutation in Rtf1 domain I that converts the RTS1 element into a replication barrier of the opposite polarity. Together our data establish that multiple protein DNA and protein-protein interactions between Rtf1 molecules and both the repeated motifs and the enhancer region of RTS1 are required for site-specific termination at the RTS1 element.
