Reference - PMID:19217403 - Fission yeast Scm3 mediates stable assembly of Cnp1/CENP-A into centromeric chromatin.
Reference summary
- PubMed ID
- PMID:19217403
- Title
- Fission yeast Scm3 mediates stable assembly of Cnp1/CENP-A into centromeric chromatin.
- Authors
- Williams JS, Hayashi T, Yanagida M, Russell P
- Citation
- Mol Cell 2009 Feb 13;33(3):287-98
- Publication year
- 2009
- Abstract
- Mis16 and Mis18 are subunits of a protein complex required for incorporation of the histone H3 variant CenH3 (Cnp1/CENP-A) into centromeric chromatin in Schizosaccharomyces pombe and mammals. How the Mis16-Mis18 complex performs this function is unknown. Here, we report that the Mis16-Mis18 complex is required for centromere localization of Scm3(Sp), a Cnp1-binding protein related to Saccharomyces cerevisiae Scm3. Scm3(Sp) is required for centromeric localization of Cnp1, while Scm3(Sp) localizes at centromeres independently of Cnp1. Like the Mis16-Mis18 complex but unlike Cnp1, Scm3(Sp) dissociates from centromeres during mitosis. Inactivation of Scm3(Sp) or Mis18 increases centromere localization of histones H3 and H2A/H2B, which are largely absent from centromeres in wild-type cells. Whereas S. cerevisiae Scm3 is proposed to replace histone H2A/H2B in centromeric nucleosomes, the dynamic behavior of S. pombe Scm3 suggests that it acts as a Cnp1 assembly/maintenance factor that directly mediates the stable deposition of Cnp1 into centromeric chromatin.
