Reference - PMID:19416828 - Schizosaccharomyces pombe Rtf2 mediates site-specific replication termination by inhibiting replication restart.
Reference summary
- PubMed ID
- PMID:19416828
- Title
- Schizosaccharomyces pombe Rtf2 mediates site-specific replication termination by inhibiting replication restart.
- Authors
- Inagawa T, Yamada-Inagawa T, Eydmann T, Mian IS, Wang TS, Dalgaard JZ
- Citation
- Proc Natl Acad Sci U S A 2009 May 12;106(19):7927-32
- Publication year
- 2009
- Abstract
- Here, we identify a phylogenetically conserved Schizosaccharomyces pombe factor, named Rtf2, as a key requirement for efficient replication termination at the site-specific replication barrier RTS1. We show that Rtf2, a proliferating cell nuclear antigen-interacting protein, promotes termination at RTS1 by preventing replication restart; in the absence of Rtf2, we observe the establishment of "slow-moving" Srs2-dependent replication forks. Analysis of the pmt3 (SUMO) and rtf2 mutants establishes that pmt3 causes a reduction in RTS1 barrier activity, that rtf2 and pmt3 are nonadditive, and that pmt3 (SUMO) partly suppresses the rtf2-dependent replication restart. Our results are consistent with a model in which Rtf2 stabilizes the replication fork stalled at RTS1 until completion of DNA synthesis by a converging replication fork initiated at a flanking origin.
