Reference - PMID:19713940 - Fission yeast IQGAP arranges actin filaments into the cytokinetic contractile ring.
Reference summary
- PubMed ID
- PMID:19713940
- Title
- Fission yeast IQGAP arranges actin filaments into the cytokinetic contractile ring.
- Authors
- Takaine M, Numata O, Nakano K
- Citation
- EMBO J 2009 Oct 21;28(20):3117-31
- Publication year
- 2009
- Abstract
- The contractile ring (CR) consists of bundled actin filaments and myosin II; however, the actin-bundling factor remains elusive. We show that the fission yeast Schizosaccharomyces pombe IQGAP Rng2 is involved in the generation of CR F-actin and required for its arrangement into a ring. An N-terminal fragment of Rng2 is necessary for the function of Rng2 and is localized to CR F-actin. In vitro the fragment promotes actin polymerization and forms linear arrays of F-actin, which are resistant to the depolymerization induced by the actin-depolymerizing factor Adf1. Our findings indicate that Rng2 is involved in the generation of CR F-actin and simultaneously bundles the filaments and regulates its dynamics by counteracting the effects of Adf1, thus enabling the reconstruction of CR F-actin bundles, which provides an insight into the physical properties of the building blocks that comprise the CR.
