Reference - PMID:19910462 - Histone variant H2A.Z regulates centromere silencing and chromosome segregation in fission yeast.
Reference summary
- PubMed ID
- PMID:19910462
- Title
- Histone variant H2A.Z regulates centromere silencing and chromosome segregation in fission yeast.
- Authors
- Hou H, Wang Y, Kallgren SP, Thompson J, Yates JR, Jia S
- Citation
- J Biol Chem 2010 Jan 15;285(3):1909-18
- Publication year
- 2010
- Abstract
- The incorporation of histone variant H2A.Z into nucleosomes plays essential roles in regulating chromatin structure and gene expression. A multisubunit complex containing chromatin remodeling protein Swr1 is responsible for the deposition of H2A.Z in budding yeast and mammals. Here, we show that the JmjC domain protein Msc1 is a novel component of the fission yeast Swr1 complex and is required for Swr1-mediated incorporation of H2A.Z into nucleosomes at gene promoters. Loss of Msc1, Swr1, or H2A.Z results in loss of silencing at centromeres and defective chromosome segregation, although centromeric levels of CENP-A, a centromere-specific histone H3 variant that is required for setting up the chromatin structure at centromeres, remain unchanged. Intriguingly, H2A.Z is required for the expression of another centromere protein, CENP-C, and overexpression of CENP-C rescues centromere silencing defects associated with H2A.Z loss. These results demonstrate the importance of H2A.Z and CENP-C in maintaining a silenced chromatin state at centromeres.
