Reference - PMID:19913479 - Cryo-EM reveals promoter DNA binding and conformational flexibility of the general transcription factor TFIID.
Reference summary
- PubMed ID
- PMID:19913479
- Title
- Cryo-EM reveals promoter DNA binding and conformational flexibility of the general transcription factor TFIID.
- Authors
- Elmlund H, Baraznenok V, Linder T, Szilagyi Z, Rofougaran R, Hofer A, Hebert H, Lindahl M, Gustafsson CM
- Citation
- Structure 2009 Nov 11;17(11):1442-52
- Publication year
- 2009
- Abstract
- The general transcription factor IID (TFIID) is required for initiation of RNA polymerase II-dependent transcription at many eukaryotic promoters. TFIID comprises the TATA-binding protein (TBP) and several conserved TBP-associated factors (TAFs). Recognition of the core promoter by TFIID assists assembly of the preinitiation complex. Using cryo-electron microscopy in combination with methods for ab initio single-particle reconstruction and heterogeneity analysis, we have produced density maps of two conformational states of Schizosaccharomyces pombe TFIID, containing and lacking TBP. We report that TBP-binding is coupled to a massive histone-fold domain rearrangement. Moreover, docking of the TBP-TAF1(N-terminus) atomic structure to the TFIID map and reconstruction of a TAF-promoter DNA complex helps to account for TAF-dependent regulation of promoter-TBP and promoter-TAF interactions.
