Reference - PMID:19940942 - Expression of the atf1+ gene is upregulated in fission yeast under nitrosative and nutritional stresses.

Reference summary

PubMed ID

PMID:19940942

Title

Expression of the atf1+ gene is upregulated in fission yeast under nitrosative and nutritional stresses.

Authors

Song SH, Kim BM, Lim CJ, Song YS, Park EH

Citation

Can J Microbiol 2009 Nov;55(11):1323-7

Publication year

2009

Abstract

This work was designed to assess regulation of the atf1+ gene in the fission yeast Schizosaccharomyces pombe under nitrosative and nutritional stresses, using the atf1+-lacZ fusion gene and RT-PCR. Nitric oxide (NO)-generating sodium nitroprusside (SNP; 10 micromol/L) and nitrogen depletion significantly enhanced synthesis of beta-galactosidase from the atf1+-lacZ fusion gene in S. pombe Pap1-positive KP1 cells, but not in S. pombe Pap1-negative TP108-3C cells. SNP (10 micromol/L) and nitrogen depletion also caused a significant increase in atf1+ mRNA levels in Pap1-positive cells, but not in Pap1-negative cells. Depletion of glucose marginally increased synthesis of beta-galactosidase from the fusion gene in S. pombe Pap1-positive cells. Taken together, the S. pombe atf1+ gene is upregulated by nitrosative and nutritional stresses on a transcriptional level, possibly via the mediation of Pap1.

Annotation