PomBase - Reference - PMID:19942659 - Characterization of two different types of UDP-glucose/-galactose 4-epimerase involved in galactosylation in fission yeast. - The Schizosaccharomyces pombe genome database

Reference summary

PubMed ID: PMID:19942659
Title: Characterization of two different types of UDP-glucose/-galactose 4-epimerase involved in galactosylation in fission yeast.
Authors: Suzuki S, Matsuzawa T, Nukigi Y, Takegawa K, Tanaka N
Citation: Microbiology (Reading) 2010 Mar;156(Pt 3):708-718
Publication year: 2010
Abstract: Schizosaccharomyces species are currently the only known organisms with two types of genes encoding UDP-glucose/-galactose 4-epimerase, uge1(+) and gal10(+). A strain deleted for uge1(+) exhibited a severe galactosylation defect and a decrease in activity and in UDP-galactose content when grown in glucose-rich medium (2 % glucose), indicating that Uge1p is a major UDP-glucose/-galactose 4-epimerase under these growth conditions. In contrast, gal10(+) was efficiently expressed and involved in galactosylation of cell-surface proteins in low-glucose medium (0.1 % glucose and 2 % glycerol), but not in galactose-containing medium. In a uge1Deltagal10Delta strain, the galactosylation defect was suppressed and UDP-galactose content restored to wild-type levels in galactose-containing medium. Disruption of gal7(+), encoding galactose-1-phosphate uridylyltransferase, in the uge1Deltagal10Delta strain reversed suppression of the galactosylation defect and reduced levels of UDP-galactose, indicating that galactose is transported from the medium to the cytosol and is converted into UDP-galactose via galactose 1-phosphate by Gal7p in Sch. pombe.