Reference - PMID:20007600 - The N-terminus of Prp1 (Prp6/U5-102 K) is essential for spliceosome activation in vivo.
Reference summary
- PubMed ID
- PMID:20007600
- Title
- The N-terminus of Prp1 (Prp6/U5-102 K) is essential for spliceosome activation in vivo.
- Authors
- Lützelberger M, Bottner CA, Schwelnus W, Zock-Emmenthal S, Razanau A, Käufer NF
- Citation
- Nucleic Acids Res 2010 Mar;38(5):1610-22
- Publication year
- 2010
- Abstract
- The spliceosomal protein Prp1 (Prp6/U5-102 K) is necessary for the integrity of pre-catalytic spliceosomal complexes. We have identified a novel regulatory function for Prp1. Expression of mutations in the N-terminus of Prp1 leads to the accumulation of pre-catalytic spliceosomal complexes containing the five snRNAs U1, U2, U5 and U4/U6 and pre-mRNAs. The mutations in the N-terminus, which prevent splicing to occur, include in vitro and in vivo identified phosphorylation sites of Prp4 kinase. These sites are highly conserved in the human ortholog U5-102 K. The results presented here demonstrate that structural integrity of the N-terminus is required to mediate a splicing event, but is not necessary for the assembly of spliceosomes.
