Reference - PMID:21984208 - Crystal structures of aprataxin ortholog Hnt3 reveal the mechanism for reversal of 5'-adenylated DNA.
Reference summary
- PubMed ID
- PMID:21984208
- Title
- Crystal structures of aprataxin ortholog Hnt3 reveal the mechanism for reversal of 5'-adenylated DNA.
- Authors
- Gong Y, Zhu D, Ding J, Dou CN, Ren X, Gu L, Jiang T, Wang DC
- Citation
- Nat Struct Mol Biol 2011 Oct 09;18(11):1297-9
- Publication year
- 2011
- Abstract
- Aprataxin is a DNA deadenylase that resolves DNA 5'-AMP termini and reverses abortive DNA ligation. The crystal structures of Schizosaccharomyces pombe aprataxin Hnt3 in its apo form and in complex to dsDNA and dsDNA-AMP reveal how Hnt3 recognizes and processes 5'-adenylated DNA in a structure-specific manner. The bound DNA adopts a 5'-flap conformation that facilitates 5'-AMP access to the active site, where AMP cleavage occurs by a canonical catalytic mechanism.
