Reference - PMID:22036784 - Purification and functional inactivation of the fission yeast MCM(MCM-BP) complex.
Reference summary
- PubMed ID
- PMID:22036784
- Title
- Purification and functional inactivation of the fission yeast MCM(MCM-BP) complex.
- Authors
- Li JJ, Schnick J, Hayles J, MacNeill SA
- Citation
- FEBS Lett 2011 Dec 15;585(24):3850-5
- Publication year
- 2011
- Abstract
- The MCM (mini-chromosome maintenance) complex is the core of the eukaryotic replicative helicase and comprises six proteins, Mcm2-Mcm7. In humans, a variant form of the complex has Mcm2 replaced by the MCM-BP protein. Recent results suggest that a similar complex exists in fission yeast with an essential role in DNA replication and cell cycle progression. Here, we describe the purification and subunit composition of the fission yeast MCM(Mcb1) complex. Using newly generated temperature-sensitive alleles, we show that loss of MCM(Mcb1) function leads to accumulation of DNA damage, checkpoint activation and cell cycle arrest, and provide evidence for a role for MCM(Mcb1) in meiosis.
