Reference - PMID:22081013 - The Chp1-Tas3 core is a multifunctional platform critical for gene silencing by RITS.
Reference summary
- PubMed ID
- PMID:22081013
- Title
- The Chp1-Tas3 core is a multifunctional platform critical for gene silencing by RITS.
- Authors
- Schalch T, Job G, Shanker S, Partridge JF, Joshua-Tor L
- Citation
- Nat Struct Mol Biol 2011 Nov 13;18(12):1351-7
- Publication year
- 2011
- Abstract
- RNA interference (RNAi) is critical for the assembly of heterochromatin at Schizosaccharomyces pombe centromeres. Central to this process is the RNA-induced initiation of transcriptional gene silencing (RITS) complex, which physically anchors small noncoding RNAs to chromatin. RITS includes Ago1, the chromodomain protein Chp1, and Tas3, which forms a bridge between Chp1 and Ago1. Chp1 is a large protein with no recognizable domains, apart from its chromodomain. Here we describe how the structured C-terminal half of Chp1 binds the Tas3 N-terminal domain, revealing the tight association of Chp1 and Tas3. The structure also shows a PIN domain at the C-terminal tip of Chp1 that controls subtelomeric transcripts through a post-transcriptional mechanism. We suggest that the Chp1-Tas3 complex provides a solid and versatile platform to recruit both RNAi-dependent and RNAi-independent gene-silencing pathways for locus-specific regulation of heterochromatin.
