Reference - PMID:22936388 - Isolation of a novel rmn1 gene genetically linked to spnab2 with respect to mRNA export in fission yeast.
Reference summary
- PubMed ID
- PMID:22936388
- Title
- Isolation of a novel rmn1 gene genetically linked to spnab2 with respect to mRNA export in fission yeast.
- Authors
- Cho YS, Jang S, Yoon JH
- Citation
- Mol Cells 2012 Sep;34(3):315-21
- Publication year
- 2012
- Abstract
- In fission yeast, Schizosaccharomyces pombe, the spnab2 gene encodes an ortholog of the budding yeast nuclear abundant poly(A)(+) RNA-binding protein 2 (Nab2) that is an essential protein required for both mRNA biogenesis and nuclear export of mRNA to the cytoplasm. We have previously isolated three mutants (SLnab1-3) that showed synthetic lethality under the repressed condition of spnab2 expression. In this study, we isolated a novel rmn1 gene as a multicopy suppressor that complemented the defects in growth and mRNA export of SLnab1 mutant cells. The rmn1 gene contained three introns and encoded a 589 amino-acid protein with the RNA recognition motif (RRM) in the central region. The Δrmn1 null mutant was viable but showed a s light mRNA export defect. However, its over-expression caused a deleterious effect on growth accompanied by intense accumulation of poly(A)(+) RNA in the nucleus. The combination of Δrmn1 with Δspnab2 or Δspmex67 also inhibited growth. In addition, Rmn1p was associated with Rae1p in vivo. These results suggest that rmn1 is a novel gene that is functionally linked to spnab2.
