Reference - PMID:23089178 - Sim3 shares some common roles with the histone chaperone Asf1 in fission yeast.
Reference summary
- PubMed ID
- PMID:23089178
- Title
- Sim3 shares some common roles with the histone chaperone Asf1 in fission yeast.
- Authors
- Tanae K, Horiuchi T, Yamakawa T, Matsuo Y, Kawamukai M
- Citation
- FEBS Lett 2012 Nov 30;586(23):4190-6
- Publication year
- 2012
- Abstract
- An H3/H4 histone chaperone, Asf1, plays an essential role in maintaining genomic stability in many species, including fission yeast. Here, we showed that overexpression of a CENP-A chaperone Sim3 suppressed the temperature sensitive phenotype of asf1-33 and asf1-30 mutants and the defect in chromatin structure, and prevented the accumulation of DNA damage in asf1-33 mutants at high temperatures. Furthermore, asf1-33 and Δsim3 were synthetic lethal. Consistent with this, shutdown of sim3 expression in asf1-33 Δsim3 double mutants that contained extragenic sim3 resulted in growth retardation. In addition, the Δsim3 mutant displayed sensitivity to thiabendazol and hydroxyurea, which suggests that Sim3 plays a general role in maintaining chromatin structure. Our results suggest a possibility that Sim3 functions as a histone chaperone.
