Reference - PMID:23254763 - Ght2⁺ is required for UDP-galactose synthesis from extracellular galactose by Schizosaccharomyces pombe.
Reference summary
- PubMed ID
- PMID:23254763
- Title
- Ght2⁺ is required for UDP-galactose synthesis from extracellular galactose by Schizosaccharomyces pombe.
- Authors
- Matsuzawa T, Hara F, Tanaka N, Tohda H, Takegawa K
- Citation
- Appl Microbiol Biotechnol 2013 Jun;97(11):4957-64
- Publication year
- 2013
- Abstract
- Schizosaccharomyces pombe has eight hexose transporter genes, ght1 (+) to ght8 (+). Here we report that ght2 (+), which is highly expressed in the presence of glucose, is essential for UDP-galactose synthesis from extracellular galactose when cells grow on glucose. The galactosylation defect of a uge1Δ mutant defective in synthesis of UDP-galactose from glucose was suppressed in galactose-containing medium, but disruption of ght2 (+) in the uge1Δ mutant reversed suppression of the galactosylation defect. Expression of Saccharomyces cerevisiae GAL2 in uge1Δght2Δ cells suppressed the defective galactosylation phenotype in galactose-containing medium. These results indicate that galactose is transported from the medium to the cytosol in a Ght2-dependent manner, and is then converted into UDP-galactose.
