Reference - PMID:23422075 - The fission yeast Pvg1p has galactose-specific pyruvyltransferase activity.
Reference summary
- PubMed ID
- PMID:23422075
- Title
- The fission yeast Pvg1p has galactose-specific pyruvyltransferase activity.
- Authors
- Yoritsune K, Matsuzawa T, Ohashi T, Takegawa K
- Citation
- FEBS Lett 2013 Apr 02;587(7):917-21
- Publication year
- 2013
- Abstract
- N-Glycan from the fission yeast Schizosaccharomyces pombe contains outer-chain pyruvic acid 4,6-ketal-linked galactose (PvGal). Here, we characterized a putative S. pombe pyruvyltransferase, Pvg1p, reported to be essential for biosynthesis of PvGal. When p-nitrophenyl-β-Gal (pNP-β-Gal) was used as a substrate, the structure of the recombinant Pvg1p product was determined to be pNP-PvGal by one- and two-dimensional NMR spectroscopy. The recombinant Pvg1p transferred pyruvyl residues from phosphoenolpyruvate specifically to β-linked galactose.
