Reference - PMID:23754748 - Splicing functions and global dependency on fission yeast slu7 reveal diversity in spliceosome assembly.
Reference summary
- PubMed ID
- PMID:23754748
- Title
- Splicing functions and global dependency on fission yeast slu7 reveal diversity in spliceosome assembly.
- Authors
- Banerjee S, Khandelia P, Melangath G, Bashir S, Nagampalli V, Vijayraghavan U
- Citation
- Mol Cell Biol 2013 Aug;33(16):3125-36
- Publication year
- 2013
- Abstract
- The multiple short introns in Schizosaccharomyces pombe genes with degenerate cis sequences and atypically positioned polypyrimidine tracts make an interesting model to investigate canonical and alternative roles for conserved splicing factors. Here we report functions and interactions of the S. pombe slu7(+) (spslu7(+)) gene product, known from Saccharomyces cerevisiae and human in vitro reactions to assemble into spliceosomes after the first catalytic reaction and to dictate 3' splice site choice during the second reaction. By using a missense mutant of this essential S. pombe factor, we detected a range of global splicing derangements that were validated in assays for the splicing status of diverse candidate introns. We ascribe widespread, intron-specific SpSlu7 functions and have deduced several features, including the branch nucleotide-to-3' splice site distance, intron length, and the impact of its A/U content at the 5' end on the intron's dependence on SpSlu7. The data imply dynamic substrate-splicing factor relationships in multiintron transcripts. Interestingly, the unexpected early splicing arrest in spslu7-2 revealed a role before catalysis. We detected a salt-stable association with U5 snRNP and observed genetic interactions with spprp1(+), a homolog of human U5-102k factor. These observations together point to an altered recruitment and dependence on SpSlu7, suggesting its role in facilitating transitions that promote catalysis, and highlight the diversity in spliceosome assembly.
