Reference - PMID:24710126 - The 19S proteasome subunit Rpt3 regulates distribution of CENP-A by associating with centromeric chromatin.
Reference summary
- PubMed ID
- PMID:24710126
- Title
- The 19S proteasome subunit Rpt3 regulates distribution of CENP-A by associating with centromeric chromatin.
- Authors
- Kitagawa T, Ishii K, Takeda K, Matsumoto T
- Citation
- Nat Commun 2014 Apr 07;5:3597
- Publication year
- 2014
- Abstract
- CENP-A, a variant of histone H3, is incorporated into centromeric chromatin and plays a role during kinetochore establishment. In fission yeast, the localization of CENP-A is limited to a region spanning 10-20 kb of the core domain of the centromere. Here, we report a mutant (rpt3-1) in which this region is expanded to 40-70 kb. Likely due to abnormal distribution of CENP-A, this mutant exhibits chromosome instability and enhanced gene silencing. Interestingly, the rpt3(+) gene encodes a subunit of the 19S proteasome, which localizes to the nuclear membrane. Although Rpt3 associates with centromeric chromatin, the mutant protein has lost this localization. A loss of the cut8(+) gene encoding an anchor of the proteasome to the nuclear membrane causes similar phenotypes as observed in the rpt3-1 mutant. Thus, we propose that the proteasome (or its subcomplex) associates with centromeric chromatin and regulates distribution of CENP-A.
