Reference - PMID:26137436 - Uncleavable Nup98-Nup96 is functional in the fission yeast Schizosaccharomyces pombe.
Reference summary
- PubMed ID
- PMID:26137436
- Title
- Uncleavable Nup98-Nup96 is functional in the fission yeast Schizosaccharomyces pombe.
- Authors
- Asakawa H, Mori C, Ohtsuki C, Iwamoto M, Hiraoka Y, Haraguchi T
- Citation
- FEBS Open Bio 2015;5:508-14
- Publication year
- 2015
- Abstract
- Essential nucleoporins Nup98 and Nup96 are coded by a single open reading frame, and produced by autopeptidase cleavage. The autocleavage site of Nup98-Nup96 is highly conserved in a wide range of organisms. To understand the importance of autocleavage, we examined a mutant that produces the Nup98-Nup96 joint molecule as a sole protein product of the nup189 (+) gene in the fission yeast Schizosaccharomyces pombe. Cells expressing only the joint molecule were found to be viable. This result indicates that autocleavage of Nup98-Nup96 is dispensable for cell growth, at least under normal culture conditions in S. pombe.
