Reference - PMID:26749213 - The AP-2 complex is required for proper temporal and spatial dynamics of endocytic patches in fission yeast.
Reference summary
- PubMed ID
- PMID:26749213
- Title
- The AP-2 complex is required for proper temporal and spatial dynamics of endocytic patches in fission yeast.
- Authors
- de León N, Hoya M, Curto MA, Moro S, Yanguas F, Doncel C, Valdivieso MH
- Citation
- Mol Microbiol 2016 May;100(3):409-24
- Publication year
- 2016
- Abstract
- In metazoans the AP-2 complex has a well-defined role in clathrin-mediated endocytosis. By contrast, its direct role in endocytosis in unicellular eukaryotes has been questioned. Here, we report co- immunoprecipitation between the fission yeast AP-2 component Apl3p and clathrin, as well as the genetic interactions between apl3Δ and clc1 and sla2Δ/end4Δ mutants. Furthermore, a double clc1 apl3Δ mutant was found to be defective in FM4-64 uptake. In an otherwise wild-type strain, apl3Δ cells exhibit altered dynamics of the endocytic sites, with a heterogeneous and extended lifetime of early and late markers at the patches. Additionally, around 50% of the endocytic patches exhibit abnormal spatial dynamics, with immobile patches and patches that bounce backwards to the cell surface, showing a pervasive effect of the absence of AP-2. These alterations in the endocytic machinery result in abnormal cell wall synthesis and morphogenesis. Our results complement those found in budding yeast and confirm that a direct role of AP-2 in endocytosis has been conserved throughout evolution.
