Reference - PMID:26877082 - ER-PM Contacts Define Actomyosin Kinetics for Proper Contractile Ring Assembly.
Reference summary
- PubMed ID
- PMID:26877082
- Title
- ER-PM Contacts Define Actomyosin Kinetics for Proper Contractile Ring Assembly.
- Authors
- Zhang D, Bidone TC, Vavylonis D
- Citation
- Curr Biol 2016 Mar 07;26(5):647-53
- Publication year
- 2016
- Abstract
- The cortical endoplasmic reticulum (ER), an elaborate network of tubules and cisternae [1], establishes contact sites with the plasma membrane (PM) through tethering machinery involving a set of conserved integral ER proteins [2]. The physiological consequences of forming ER-PM contacts are not fully understood. Here, we reveal a kinetic restriction role of ER-PM contacts over ring compaction process for proper actomyosin ring assembly in Schizosaccharomyces pombe. We show that fission yeast cells deficient in ER-PM contacts exhibit aberrant equatorial clustering of actin cables during ring assembly and are particularly susceptible to compromised actin filament crosslinking activity. Using quantitative image analyses and computer simulation, we demonstrate that ER-PM contacts function to modulate the distribution of ring components and to constrain their compaction kinetics. We propose that ER-PM contacts have evolved as important physical modulators to ensure robust ring assembly.
