Reference - PMID:26890608 - A functional genome-wide genetic screening identifies new pathways controlling the G1/S transcriptional wave.
Reference summary
- PubMed ID
- PMID:26890608
- Title
- A functional genome-wide genetic screening identifies new pathways controlling the G1/S transcriptional wave.
- Authors
- Gaspa L, González-Medina A, Hidalgo E, Ayté J
- Citation
- Cell Cycle 2016;15(5):720-9
- Publication year
- 2016
- Abstract
- The Schizosaccharomyces pombe MBF complex activates the transcription of genes required for DNA synthesis and S phase. The MBF complex contains several proteins, including the core components Cdc10, Res1 and Res2, the co-repressor proteins Yox1 and Nrm1 and the co-activator Rep2. It has recently been shown how MBF is regulated when either the DNA damage or the DNA synthesis checkpoints are activated. However, how MBF is regulated in a normal unperturbed cell cycle is still not well understood. We have set up a genome-wide genomic screen searching for global regulators of MBF. We have crossed our knock-out collection library with a reporter strain that allows the measurement of MBF activity in live cells by flow cytometry. We confirm previously known regulators of MBF and show that COP9/signalosome and tRNA methyltransferases also regulate MBF activity.
