Reference - PMID:27146110 - Kinesin-8 effects on mitotic microtubule dynamics contribute to spindle function in fission yeast.
Reference summary
- PubMed ID
- PMID:27146110
- Title
- Kinesin-8 effects on mitotic microtubule dynamics contribute to spindle function in fission yeast.
- Authors
- Gergely ZR, Crapo A, Hough LE, McIntosh JR, Betterton MD
- Citation
- Mol Biol Cell 2016 Nov 07;27(22):3490-3514
- Publication year
- 2016
- Abstract
- Kinesin-8 motor proteins destabilize microtubules. Their absence during cell division is associated with disorganized mitotic chromosome movements and chromosome loss. Despite recent work studying effects of kinesin-8s on microtubule dynamics, it remains unclear whether the kinesin-8 mitotic phenotypes are consequences of their effect on microtubule dynamics, their well-established motor activity, or additional, unknown functions. To better understand the role of kinesin-8 proteins in mitosis, we studied the effects of deletion of the fission yeast kinesin-8 proteins Klp5 and Klp6 on chromosome movements and spindle length dynamics. Aberrant microtubule-driven kinetochore pushing movements and tripolar mitotic spindles occurred in cells lacking Klp5 but not Klp6. Kinesin-8-deletion strains showed large fluctuations in metaphase spindle length, suggesting a disruption of spindle length stabilization. Comparison of our results from light microscopy with a mathematical model suggests that kinesin-8-induced effects on microtubule dynamics, kinetochore attachment stability, and sliding force in the spindle can explain the aberrant chromosome movements and spindle length fluctuations seen.
