Reference - PMID:28784611 - Phosphoinositide-mediated ring anchoring resists perpendicular forces to promote medial cytokinesis.
Reference summary
- PubMed ID
- PMID:28784611
- Title
- Phosphoinositide-mediated ring anchoring resists perpendicular forces to promote medial cytokinesis.
- Authors
- Snider CE, Willet AH, Chen JS, Arpağ G, Zanic M, Gould KL
- Citation
- J Cell Biol 2017 Oct 02;216(10):3041-3050
- Publication year
- 2017
- Abstract
- Many eukaryotic cells divide by assembling and constricting an actin- and myosin-based contractile ring (CR) that is physically linked to the plasma membrane (PM). In this study, we report that Schizosaccharomyces pombe cells lacking efr3 , which encodes a conserved PM scaffold for the phosphatidylinositol-4 kinase Stt4, build CRs that can slide away from the cell middle during anaphase in a myosin V-dependent manner. The Efr3-dependent CR-anchoring mechanism is distinct from previously reported pathways dependent on the Fes/CIP4 homology Bin-Amphiphysin-Rvs167 (F-BAR) protein Cdc15 and paxillin Pxl1. In efr3Δ , the concentrations of several membrane-binding proteins were reduced in the CR and/or on the PM. Our results suggest that proper PM lipid composition is important to stabilize the central position of the CR and resist myosin V-based forces to promote the fidelity of cell division.
