Reference - PMID:28904333 - The Ino80 complex mediates epigenetic centromere propagation via active removal of histone H3.
Reference summary
- PubMed ID
- PMID:28904333
- Title
- The Ino80 complex mediates epigenetic centromere propagation via active removal of histone H3.
- Authors
- Choi ES, Cheon Y, Kang K, Lee D
- Citation
- Nat Commun 2017 Sep 13;8(1):529
- Publication year
- 2017
- Abstract
- The centromere is the chromosomal locus at which the kinetochore is assembled to direct chromosome segregation. The histone H3 variant, centromere protein A (CENP-A), is known to epigenetically mark active centromeres, but the mechanism by which CENP-A propagates at the centromere, replacing histone H3, remains poorly understood. Using fission yeast, here we show that the Ino80 adenosine triphosphate (ATP)-dependent chromatin-remodeling complex, which removes histone H3-containing nucleosomes from associated chromatin, promotes CENP-A Cnp1 chromatin assembly at the centromere in a redundant manner with another chromatin-remodeling factor Chd1 Hrp1 . CENP-A Cnp1 chromatin actively recruits the Ino80 complex to centromeres to elicit eviction of histone H3-containing nucleosomes. Artificial targeting of Ino80 subunits to a non-centromeric DNA sequence placed in a native centromere enhances the spreading of CENP-A Cnp1 chromatin into the non-centromeric DNA. Based on these results, we propose that CENP-A Cnp1 chromatin employs the Ino80 complex to mediate the replacement of histone H3 with CENP-A Cnp1 , and thereby reinforces itself.The histone variant CENP-A marks active centromeres and replaces H3 at centromeres through a poorly understood mechanism. Here, the authors provide evidence that the chromatin remodeller Ino80 promotes CENP-A chromatin assembly at the centromere in fission yeast.
