Reference - PMID:29453312 - Inhibition of Ras activity coordinates cell fusion with cell-cell contact during yeast mating.
Reference summary
- PubMed ID
- PMID:29453312
- Title
- Inhibition of Ras activity coordinates cell fusion with cell-cell contact during yeast mating.
- Authors
- Merlini L, Khalili B, Dudin O, Michon L, Vincenzetti V, Martin SG
- Citation
- J Cell Biol 2018 Apr 02;217(4):1467-1483
- Publication year
- 2018
- Abstract
- In the fission yeast Schizosaccharomyces pombe , pheromone signaling engages a signaling pathway composed of a G protein-coupled receptor, Ras, and a mitogen-activated protein kinase (MAPK) cascade that triggers sexual differentiation and gamete fusion. Cell-cell fusion requires local cell wall digestion, which relies on an initially dynamic actin fusion focus that becomes stabilized upon local enrichment of the signaling cascade on the structure. We constructed a live-reporter of active Ras1 (Ras1-guanosine triphosphate [GTP]) that shows Ras activity at polarity sites peaking on the fusion structure before fusion. Remarkably, constitutive Ras1 activation promoted fusion focus stabilization and fusion attempts irrespective of cell pairing, leading to cell lysis. Ras1 activity was restricted by the guanosine triphosphatase-activating protein Gap1, which was itself recruited to sites of Ras1-GTP and was essential to block untimely fusion attempts. We propose that negative feedback control of Ras activity restrains the MAPK signal and couples fusion with cell-cell engagement.
