Reference - PMID:30099677 - Genome-wide evidences of bisphenol a toxicity using Schizosaccharomyces pombe.
Reference summary
- PubMed ID
- PMID:30099677
- Title
- Genome-wide evidences of bisphenol a toxicity using Schizosaccharomyces pombe.
- Authors
- Kim DM, Heo J, Lee DW, Tsuji M, Yang M
- Citation
- Arch Pharm Res 2018 Aug;41(8):830-837
- Publication year
- 2018
- Abstract
- To clarify reliable toxic mechanisms of bisphenol A (BPA), an endocrine disrupting chemical, we approached an alternative animal and whole genome analyses with the yeast knockout library (YKO) of Schizosaccharomyces pombe. As results, the 50% growth inhibition concentrations (GI 50 ) of BPA was approximately 600 μM and the YKO-three step screening revealed the top 10 target candidate genes including dbp2, utp18, srs1, tif224, use1, qcr1, etc. The screening results were confirmed in human embryonic stem cell (hES)-derived hepatic cells and HepG2 human liver cancer cells. We found BPA down-regulated UQCRC, the human orthlog of S. pombe- qcr1, as a part of the mitochondrial respiratory chain, in HepG2 cells and hESs during cell differentiation into hepatic cells. Therefore, BPA may induce mitochondrial dysfunction and disruption of differentiation by suppressing UQCRC1.
