Reference - PMID:31042107 - Generation of temperature sensitive mutations with error-prone PCR in a gene encoding a component of the spindle pole body in fission yeast.

Reference summary

PubMed ID

PMID:31042107

Title

Generation of temperature sensitive mutations with error-prone PCR in a gene encoding a component of the spindle pole body in fission yeast.

Authors

Tang NH, Fong CS, Masuda H, Jourdain I, Yukawa M, Toda T

Citation

Biosci Biotechnol Biochem 2019 Sep;83(9):1717-1720

Publication year

2019

Abstract

Temperature-sensitive (ts) mutants provide powerful tools for investigation of cellular functions of essential genes. We report here asimple procedure to generate ts mutations using error-prone PCR within pcp1 that encodes aspindle pole body (SPB) component in Schizosaccharomyces pombe . This manipulation is not restricted to pcp1 , and can be suited to any essential genes involved in other processes.

Annotation